A simple, precise, accurate, reproducible and economical reverse phase liquid chromatography method was developed and validated for the quantitative simultaneous estimation of lamivudine and tenofovir disoproxil fumarate in bulk and tablet dosage form. Estimation of drugs in this combination was done with a C18 column Kromasil 100-5C18 column (250 mm x 4.6 mm) using mobile phase of composition Acetonitrile and phosphate buffer (60:40 v/v, pH 6.0). The flow rate was 1 ml/min and the effluents were monitored at 266 nm. The retention time of lamivudine and tenofovir disoproxil fumarate was 3.3 min and 6.25 min respectively. The method was found to be linear over a concentration range of 20-100 g/ml for both lamivudine and tenofovir disoproxil fumarate. The established method proved as reproducible one with a %RSD value of less than 2 and having the robustness and accuracy within the specified limits. Assay of marketed formulation was determined and found with 98.1% and 97.6% for lamivudine and tenofovir disoproxil fumarate respectively. The method was validated according to the guidelines of International Conference on Harmonization (ICH) and was successfully employed in the estimation of commercial formulations. This liquid chromatographic method can be applied for the qualitative and quantitative determination of selected drugs by the modern chemist.
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